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Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Cross species quorum quenching using a native AI-2 processing enzyme.

Varnika Roy1, Rohan Fernandes, Chen-Yu Tsao

  • 1Department of Molecular and Cell Biology, University of Maryland, College Park, Maryland 20742, USA.

ACS Chemical Biology
|December 23, 2009
PubMed
Summary

Researchers developed a novel enzymatic method to block bacterial communication (quorum sensing) by targeting the autoinducer-2 molecule. This approach effectively reduces bacterial responses and offers a new strategy for controlling microbial behavior and pathogenicity.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • Bacterial quorum sensing (QS) regulates phenotypes, including pathogenicity, via cell-cell communication.
  • Interfering with QS is a promising strategy for next-generation antimicrobials.
  • The interspecies signaling molecule autoinducer-2 (AI-2) is prevalent across many bacterial species.

Purpose of the Study:

  • To develop a novel enzymatic method for interrupting bacterial QS.
  • To target the AI-2 QS system for controlling inter- and intra-species communication.
  • To investigate the potential of using native bacterial machinery for antimicrobial strategies.

Main Methods:

  • Demonstrated that Escherichia coli AI-2 kinase (LsrK) phosphorylates AI-2 in vitro.
  • Applied LsrK-treated AI-2 ex vivo to reduce QS responses in E. coli populations.
  • Tested LsrK-mediated AI-2 degradation on Salmonella typhimurium and Vibrio harveyi.
  • Evaluated the method in a synthetic ecosystem with co-cultured enteric and marine bacteria.

Main Results:

  • LsrK effectively phosphorylated AI-2 in vitro.
  • Ex vivo addition of LsrK-treated AI-2 significantly reduced QS responses in E. coli.
  • LsrK-mediated AI-2 degradation attenuated QS responses in S. typhimurium and V. harveyi.
  • Reduced inter-species QS crosstalk was observed in co-cultures of E. coli and S. typhimurium.

Conclusions:

  • A nature-inspired enzymatic approach can quench bacterial QS systems.
  • This method effectively reduces QS responses across different bacterial species and in mixed ecosystems.
  • The strategy holds potential for controlling bacterial phenotypes and pathogenicity.