Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Minimal Rev-response element for type 1 human immunodeficiency virus.

X J Huang1, T J Hope, B L Bond

  • 1Department of Pathology, University of California, San Francisco 94143-0506.

Journal of Virology
|April 1, 1991
PubMed
Summary

The Rev protein controls viral mRNA export using a specific RNA sequence. A shorter Rev-response element (RRE) fragment retains this function, showing its essential role in viral gene expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bladder health and the urogenital microbiome in community-dwelling adult females.

mSystems·2025
Same author

Influence of routine follow-up radiographs on recommendations for management of antebrachial fractures in dogs.

Australian veterinary journal·2025
Same author

Interprofessional education and collaborative practice with practicing radiographers: A mixed methods scoping review.

Radiography (London, England : 1995)·2025
Same author

Waste not, want not: measuring waste and potential clinical risk from limited gatekeeping.

Irish medical journal·2025
Same author

[Treatment strategies of acute myeloid leukemia in elderly patients].

Zhonghua nei ke za zhi·2023
Same author

[Clinical characteristics of patients with human adenovirus infection undergoing allogeneic hematopoietic stem cell transplantation].

Zhonghua nei ke za zhi·2023

Area of Science:

  • Molecular Biology
  • Virology
  • Gene Expression Regulation

Background:

  • The Rev protein is crucial for the nuclear export of specific viral messenger RNAs (mRNAs).
  • This export is mediated by a structured RNA sequence known as the Rev-response element (RRE).
  • The full-length RRE is approximately 240 bases and contains critical binding sites for the Rev protein.

Purpose of the Study:

  • To investigate the minimal sequence requirements of the RRE for Rev-mediated nuclear export.
  • To determine if a truncated RRE fragment can functionally replace the full-length RRE in vivo.
  • To analyze the efficiency of a shortened RRE in regulating viral mRNA export.

Main Methods:

  • In vivo assays were used to assess the function of truncated RRE sequences.

Related Experiment Videos

  • The study focused on an 88-base RRE mutant containing a known Rev binding site.
  • The functionality of two tandem copies of the mutant RRE was compared to the full-length RRE.

    • Main Results:

    • An 88-base truncated RRE was demonstrated to mediate Rev responsiveness effectively in vivo.
    • This shortened RRE fragment, containing a key Rev binding site, is sufficient for Rev-mediated nuclear export.
    • Two tandem copies of the mutant RRE exhibited functional efficiency comparable to the full-length RRE.

    Conclusions:

    • The Rev-response element (RRE) can be significantly truncated while retaining its ability to interact with the Rev protein.
    • A minimal 88-base RRE sequence is sufficient to confer Rev responsiveness for viral mRNA nuclear export.
    • These findings highlight the importance of specific binding sites within the RRE for efficient viral gene regulation.