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Related Experiment Video

Updated: Jun 17, 2026

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Published on: July 16, 2016

Cell therapy for diabetes: stem cells, progenitors or beta-cell replication?

L Jorge Gonez¹, Kenneth R Knight

¹The Australian Tissue Engineering Centre Ltd, 42 Fitzroy Street, Fitzroy, Victoria 3065, Australia. jgonez@unimelb.edu.au <jgonez@unimelb.edu.au>

Molecular and Cellular Endocrinology

|December 23, 2009

Summary

Type 1 diabetes (T1D) research explores regenerating insulin-producing cells to cure the disease. Studies show pancreatic stem cells, progenitors, and beta-cells can regenerate, offering hope for cell-based therapies.

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Last Updated: Jun 17, 2026

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Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

Area of Science:

Endocrinology
Regenerative Medicine
Immunology

Background:

Type 1 diabetes (T1D) necessitates restoring insulin production and preventing immune destruction of beta-cells.
Rodent models indicate potential for regenerating insulin-producing cells through various sources.
Understanding cellular and molecular mechanisms is crucial for effective T1D therapies.

Purpose of the Study:

To investigate the potential of different cell sources for insulin-producing cell regeneration in T1D.
To explore cellular and molecular mechanisms underlying beta-cell regeneration in vitro and in vivo.
To assess the behavior of potential cell therapies using a novel in vivo chamber model of T1D.

Main Methods:

Review of existing experiments in rodent models regarding pancreatic stem cells, committed progenitors, and replicating beta-cells.
In vitro and in vivo investigations into the cellular and molecular mechanisms of insulin-producing cells.
Development and utilization of a unique surgical in vivo chamber model for T1D research.

Main Results:

Evidence from rodent models suggests that pancreatic stem cells, committed progenitors, and beta-cells can contribute to insulin-producing cell regeneration.
Cellular and molecular mechanisms governing these regenerative processes have been studied.
The developed in vivo chamber model allows for the evaluation of different cell sources for T1D therapy.

Conclusions:

Regeneration of insulin-producing cells is a key strategy for a type 1 diabetes cure.
Various cell types, including stem cells and progenitors, show promise for beta-cell regeneration.
The novel in vivo model provides a platform for assessing cell-based therapies for T1D.