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Updated: Jun 17, 2026

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
Published on: July 29, 2015
Neuropotent self-renewing neural stem (NS) cells derived from mouse induced pluripotent stem (iPS) cells
Marco Onorati1, Stefano Camnasio, Maurizio Binetti
1Università degli Studi di Milano, Department of Pharmacological Sciences and Centre for Stem Cell Research, 20133 Milano, Italy.
Abstract:
Neural stem (NS) cells are a homogenous population of stem cells that expands in monolayer under serum-free conditions while remaining highly neuropotent. Here, we generated NS cells from induced pluripotent stem (iPS) cells that were previously derived from mouse fibroblasts (NS-(f)iPS). We showed that NS-(f)iPS cells exhibit long-term expansion and express markers of neurogenic radial glia. Analyses of the regional markers expressed in NS-(f)iPS cells suggested a ventral-rhombencephalic identity. Upon exposure to differentiation protocols, NS-(f)iPS cells produce neurons, astrocytes, and oligodendrocytes with an efficiency similar to ES-derived NS cells. NS-(f)iPS cells represent a new tool for studying neural cell fate determination and terminal differentiation, providing an interesting resource for experimental transplantation. Comparative studies between NS cells derived from iPS cells, reprogrammed from different somatic sources, and from authentic ES cells are necessary to identify critical elements for multipotency acquisition.
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