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Published on: June 6, 2025
Effects of sesame oil in the model of adjuvant arthritis
Ruzena Sotnikova1, Silvester Ponist, Jana Navarova
1Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia. exfarosa@savba.sk
Objectives:
The goal of this study was to evaluate the effect of sesame oil on functional damage induced by adjuvant arthritis (AA) and on changes of selected biochemical parameters reflecting oxidative tissue injury.
Design:
Mycobacterium butyricum in incomplete Freund's adjuvans was intradermally administered to Lewis male rats. Hind paw edema and endothelium-dependent relaxation of the aorta were determined on day 28. Further, plasmatic levels of TBARS, gamma-glutamyltransferase (GGT) activity in the joint and spleen tissues, level of protein carbonyls and total antioxidant capacity (TAC) in plasma, as well as activity of the lysosomal enzyme N-acetyl-glucosaminidase (NAGA) in serum were assessed. The effect of sesame oil (SO, 1ml/kg, daily oral administration) was evaluated on day 28.
Results:
The beneficial effect of sesame oil on markers of oxidative stress accompanying AA was demonstrated by decrease of plasma TBARS and decrease of GGT activity in the joint and spleen tissues. Level of protein carbonyls, TAC in plasma and activity of NAGA in serum and in the kidney were improved, yet not significantly. In the hind paw edema the maximal increase was found on day 28 of AA, and in the same time we observed a significant decrease of aortic endothelium-dependent relaxation. Administration of SO resulted in mild, non-significant decrease of hind paw swelling and in significantly increased acetylcholine-evoked relaxation.
Conclusion:
We conclude that SO has beneficial effects on oxidative stress induced biochemical changes occurring in AA, moreover it improves endothelium-dependent relaxation of the aorta and tends to decrease hind paw edema.
Insights
Sesame oil (SO) shows beneficial effects in reducing oxidative stress and improving vascular function in adjuvant arthritis (AA) rat models. This study demonstrates SO
Area of Science:
- Biochemistry
- Pharmacology
- Immunology
Background:
- Adjuvant arthritis (AA) is an inflammatory condition associated with oxidative stress and impaired vascular function.
- Oxidative tissue injury plays a significant role in the pathogenesis of AA.
Purpose of the Study:
- To investigate the therapeutic potential of sesame oil (SO) in mitigating functional damage and biochemical alterations in a rat model of adjuvant arthritis (AA).
- To assess the impact of SO on oxidative stress markers and endothelium-dependent relaxation in AA.
Main Methods:
- Adjuvant arthritis was induced in male Lewis rats using Mycobacterium butyricum.
- Rats were administered sesame oil (SO) orally at 1ml/kg daily.
- Evaluated parameters included hind paw edema, aortic endothelium-dependent relaxation, plasma TBARS, GGT activity, protein carbonyls, TAC, and NAGA activity.
Main Results:
- Sesame oil administration significantly reduced plasma TBARS and GGT activity in joint and spleen tissues.
- SO treatment led to a significant improvement in acetylcholine-evoked aortic relaxation.
- While not statistically significant, SO showed a trend towards decreasing hind paw edema and improving other oxidative stress markers.
Conclusions:
- Sesame oil exhibits beneficial effects on biochemical changes related to oxidative stress in adjuvant arthritis.
- SO improves endothelium-dependent relaxation and demonstrates a tendency to reduce paw edema in AA models.
