Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CRISPR-AuNP: physicochemical optimization of a gold nanoparticle platform for cost-effective and modular non-viral gene editing in HSPCs.

Gene therapy·2026
Same author

A functional map of CDK-drug interactions at single amino acid resolution.

bioRxiv : the preprint server for biology·2025
Same author

Towards directed therapy for fusion-positive rhabdomyosarcoma.

Pharmacology & therapeutics·2025
Same author

Small molecule modulators of TOX protein re-invigorate T cell activity.

bioRxiv : the preprint server for biology·2025
Same author

Chemical Probe Discovery for DEAD-Box RNA-Binding Protein DDX21 Using Small-Molecule Microarrays.

ACS chemical biology·2025
Same author

At The Interface: Small-Molecule Inhibitors of Soluble Cytokines.

Chemical reviews·2025

Related Experiment Video

Updated: Jun 17, 2026

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?
14:20

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?

Published on: June 13, 2014

Binding affinity and kinetic analysis of targeted small molecule-modified nanoparticles.

Carlos Tassa1, Jay L Duffner, Timothy A Lewis

  • 1Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Bioconjugate Chemistry
|December 24, 2009
PubMed
Summary

Multivalent nanoparticle targeting ligands significantly boost binding avidity, even with weak interactions. This study quantifies nanoparticle-ligand binding kinetics, informing future biomedical designs.

More Related Videos

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
11:28

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Related Experiment Videos

Last Updated: Jun 17, 2026

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?
14:20

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?

Published on: June 13, 2014

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
11:28

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Surface Chemistry

Background:

  • Surface-conjugated targeting ligands on nanoparticles are crucial for biomedical applications.
  • Multivalent conjugation is presumed to enhance nanoparticle binding, but understanding is incomplete due to complex interactions.

Purpose of the Study:

  • To quantitatively investigate the affinity and binding kinetics of nanoparticles with surface-conjugated small molecule ligands.
  • To understand how ligand conjugation affects nanoparticle binding avidity and kinetics.

Main Methods:

  • Utilized surface plasmon resonance (SPR) to directly measure nanoparticle-protein interactions.
  • Studied a series of structurally related targeting ligands with a 4500-fold affinity range.
  • Performed SPR at physiologically relevant protein densities.

Main Results:

  • Even weak small molecule ligands significantly enhanced target-specific avidity by up to 4 orders of magnitude via multivalent interactions.
  • Observed a wider range of kinetic effects than previously reported.
  • Demonstrated that quantitative SPR measurements can characterize nanoparticle binding variations.

Conclusions:

  • Multivalent interactions with nanoparticles can dramatically increase target avidity.
  • SPR provides a generalizable method for characterizing nanoparticle-ligand interactions.
  • This approach can guide the rational design of nanoparticles for biomedical applications.