Related Experiment Video
Updated: Jun 17, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
[Diversity of nirS-type denitrifying bacteria under different nitrogen fertilizer management in wheat soil]
Xuhua Mo1, Wei Ma, Rongjiu Shi
1Key Laboratory of Terrestrial Ecological Processes, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. shiricao2003@yahoo.com.cn
Objective:
To study the effect of inorganic nitrogen fertilizer on nirS-type denitrifiers' community in the wheat soil.
Methods:
We constructed nirS gene libraries of two different treatments of soil: soil fertilized with inorganic nitrogen fertilizer (B-UN) and without nitrogen fertilizer (B-NN). And we used restriction fragment length polymorphism (RFLP) method to analyze the nirS-type denitrifiers' diversity.
Results:
There were 27 operational taxa units (OTUs) in each treatment after Msp I and Afa I digestion and only nine OTUs existed in both treatments. The ecological indexes such as Shannon-Wiene index, Simpon index, richness index and evenness index of two different soils were nearly equivalent. However, significant difference was found between the OTU clusters from clone libraries of different treatments. Eleven representative clones were sequenced. The similarities of ten sequences were from 73% to 95% to the sequences of the database. There was one sequence which had no similar nucleotide identities in database.
Conclusion:
The application of inorganic nitrogen fertilizer altered significantly the nirS-type denitrifiers' community in the wheat soil.
Related Concept Videos
Inorganic Nitrogen Assimilation
The Roles of Bacteria and Fungi in Plant Nutrition
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
Microbes and the Nitrogen Cycle
Soil Microbial Ecology
Diversity of Archaea I

