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Updated: Jun 17, 2026
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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
[Isolation and identification of endophytes from Pinellia]
Jianling Liu1, Baobao Chen, Yonghong Liu
1College of Life Sciences, Northwest University, Xi'an 710069, China. ljl2003ljl@126.com
Summary
Endophytic fungi in Pinellia ternata are diverse and abundant, with variations observed across different plant organs. This study isolated 84 strains, identifying 15 genera of these beneficial microbes.
Area of Science:
- Microbiology
- Botany
- Plant Pathology
Context:
- Endophytes are microorganisms residing within plant tissues, playing crucial roles in plant health and ecosystem functions.
- Pinellia ternata is a significant medicinal plant, and understanding its associated microbial communities is vital for its cultivation and application.
Purpose:
- To investigate the diversity and distribution of endophytic fungi within various parts of Pinellia ternata.
- To identify the fungal genera and families associated with Pinellia ternata from different habitats.
Summary:
- Eighty-four endophytic fungal strains were isolated from Pinellia ternata collected from three distinct habitats.
- Morphological identification revealed the presence of 15 fungal genera belonging to 4 families, indicating a rich endophytic community.
- Significant variations in the quantity and species composition of endophytes were observed across different organs of the plant.
Impact:
- This research highlights the rich endophytic mycobiota of Pinellia ternata, providing a foundation for future studies on their functional roles.
- The findings contribute to the understanding of plant-microbe interactions and can inform strategies for enhancing the medicinal properties of Pinellia ternata through microbial management.
