Related Experiment Video
Updated: Jun 17, 2026
Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model
Published on: August 4, 2017
[Migration of intravenously injected adipose tissue-derived stem cells in SD rats with soft tissue wound]
Hua Nan1, Jian-Hua Gao, Feng Lu
1Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To explore the migration of intravenously injected adipose tissue-derived stem cells (ADSCs) in SD rats with soft tissue wound.
Methods:
1.8 cm x 1.8 cm full thickness skin defect and 0.5 cm in depth soft tissue defects were made on the back of 6 SD rats. ADSCs were isolated and cultured in vitro for 3 passages. 2.4 x 10(6) ADSCs were labeled with DiI and transplanted into the SD rats through tail vein. Normal skin and wound tissue samples were collected for fluorescent distribution observation 24, 48 days after injection, respectively.
Results:
Compared to normal skin, more fluorescent positive cells were detected in the margin and deep layer of the wound 24 days after operation. But it is accumulated within dermis and adenoid 48 days after operation.
Conclusion:
Wound can probably induce the migration and accumulation of intravenously transplanted ADSCs.
