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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

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Published on: February 23, 2020

[Fully automated immunoassay system utilizing microchip electrophoresis].

Takuma Ohtsubo¹, Isao Yamaguchi, Tatsuo Kurosawa

¹Wako Pure Chemical Industries Ltd., Osaka 540-8605, Japan.

Rinsho Byori. the Japanese Journal of Clinical Pathology

|December 25, 2009

Summary

This study presents a novel micro Total Analysis System (microTAS) for rapid and sensitive PIVKA II immunoassay. The system offers high accuracy and efficiency for hepatocellular carcinoma diagnosis.

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Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

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Published on: April 21, 2019

Area of Science:

Biomedical Engineering
Analytical Chemistry
Clinical Diagnostics

Context:

Microfluidic devices offer advantages for clinical diagnostics, including reduced reaction times and sample volumes.
Traditional immunoassays can be time-consuming and require larger sample volumes.
Microchip electrophoresis combined with isotachophoresis provides efficient separation and immunoreaction capabilities.

Purpose:

To develop a fully automated microfluidic immunoassay system for PIVKA II detection.
To evaluate the performance of the microTAS system in terms of sensitivity, reproducibility, and correlation with existing methods.
To demonstrate the utility of the developed system for clinical applications, specifically in hepatocellular carcinoma diagnosis.

Summary:

A novel microTAS system was developed using isotachophoresis and capillary gel electrophoresis on polymer microchips for a fully automated immunoassay.
Laser-Induced Fluorescence (LIF) detected sandwich immunocomplexes, demonstrating high sensitivity (LOD = 5mAU/mL) and reproducibility (CV = 1.0-5.7%) for PIVKA II.
The assay showed excellent correlation with a commercial kit (r=0.991) and a rapid turnaround time of approximately 9 minutes.

Impact:

The developed microTAS system provides a rapid, sensitive, and reproducible method for PIVKA II detection.
This technology has the potential to significantly improve the diagnosis and prognosis of hepatocellular carcinoma.
The system's efficiency and reduced reagent consumption highlight the advantages of microfluidic devices in clinical diagnostics.