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Updated: Jun 17, 2026
06:25
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Published on: July 10, 2019
[Identification of human pathogenic variola and monkeypox viruses by real-time polymerase chain reaction]
Voprosy Virusologii
|December 25, 2009
Abstract:
A kit of specific oligonucleotide primers and hybridization probes has been proposed to detect orthopoxviruses (OPV) and to discriminate human pathogenic viruses, such as variola virus and monkey virus by real-time polymerase chain reaction (PCR). For real-time PCR, the following pairs of fluorophore and a fluorescence quencher were used: TAMRA-BHQ2 for genus-specific probes and FAM-BHQ1 for species-specific ones (variola virus, monkeypox virus, ectomelia virus). The specificity of this assay was tested on 38 strains of 6 OPV species and it was 100%.
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