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Molecular characterization of class 1 integrons in MDR Pseudomonas aeruginosa isolated from clinical settings in

Fereshteh Shahcheraghi1, Farzad Badmasti, Mohammad M Feizabadi

  • 1Department of Microbiology, Pasteur Institute, Tehran, Iran. shahcheraghifereshteh@yahoo.com <shahcheraghifereshteh@yahoo.com>

FEMS Immunology and Medical Microbiology
|December 25, 2009
PubMed

Insights

Multidrug-resistant Pseudomonas aeruginosa (MDRPA) infections are a growing concern. This study identified class 1 integrons in 5.46% of isolates, revealing specific gene cassette arrays responsible for resistance.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Pseudomonas aeruginosa is a significant opportunistic pathogen.
  • The emergence of multidrug-resistant P. aeruginosa (MDRPA) poses a global health threat.
  • Class 1 integrons are key genetic elements mediating antimicrobial resistance acquisition and dissemination.

Purpose of the Study:

  • To investigate the prevalence and genetic content of class 1 integrons in clinical MDRPA isolates.
  • To characterize the gene cassette arrays within class 1 integrons in P. aeruginosa from Iranian hospitals.

Main Methods:

  • Collection of 750 clinical P. aeruginosa isolates from five hospitals (March 2007-May 2009).
  • Identification of multidrug-resistant P. aeruginosa (MDRPA) isolates.
  • Polymerase chain reaction (PCR) assays to detect class 1 integrons and amplify internal variable regions (IVRs).
  • Sequencing of amplified IVRs to identify gene cassette arrays.

Main Results:

  • Forty-one isolates (5.46%) were characterized as MDRPA.
  • Three distinct class 1 integron gene cassette arrays were identified: aadB (0.8 kb), aadA6-orfD (1.3 kb), and bla(OXA10)-aacA4 (1.7 kb).
  • These arrays showed varying distributions among the clinical isolates.

Conclusions:

  • Class 1 integrons are prevalent in MDRPA clinical isolates from Iranian hospitals.
  • A limited diversity of gene cassette arrays was observed within these class 1 integrons.
  • This study provides the first report on the gene cassette content of class 1 integrons in P. aeruginosa isolates in Iran.

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