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Related Concept Videos

Sequences01:29

Sequences

295
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
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Arithmetic Sequences01:30

Arithmetic Sequences

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An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Cis-regulatory Sequences

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No description available
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
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Next-generation sequencing.

Jorge S Reis-Filho1

  • 1Molecular Pathology Team, The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. Jorge.Reis-Filho@icr.ac.uk

Breast Cancer Research : BCR
|December 25, 2009
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Summary
This summary is machine-generated.

Next-generation sequencing (NGS) revolutionizes cancer research by enabling detailed genomic, transcriptomic, and epigenetic analysis. This technology offers unprecedented insights into cancer, presenting both opportunities and challenges for breast cancer studies.

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Area of Science:

  • Genomics
  • Transcriptomics
  • Epigenetics
  • Cancer Research

Background:

  • Next-generation sequencing (NGS), also known as massively parallel sequencing, is transforming cancer characterization.
  • Current NGS capabilities allow for comprehensive analysis of the cancer genome, transcriptome, and epigenome.
  • This technology enables base-pair resolution cataloging of mutations, copy number aberrations, and somatic rearrangements.

Purpose of the Study:

  • To discuss the potential impact of next-generation sequencing on breast cancer research.
  • To highlight the challenges associated with implementing this advanced sequencing technology in breast cancer studies.

Main Methods:

  • Massively parallel sequencing for genomic characterization.
  • Unbiased transcriptomic analysis of various RNA types (mRNAs, small RNAs, noncoding RNAs).
  • Genome-wide methylation assays.
  • High-throughput chromatin immunoprecipitation assays.

Main Results:

  • NGS allows for rapid and detailed characterization of cancer at multiple molecular levels.
  • The technology facilitates comprehensive profiling of mutations, copy number changes, and rearrangements.
  • NGS enables unbiased analysis of the entire transcriptome and epigenetic landscape.

Conclusions:

  • Next-generation sequencing holds significant promise for advancing breast cancer research.
  • Addressing the challenges of this breakthrough technology is crucial for its effective application in breast cancer studies.