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Real-time PCR for diagnosis of oculoglandular tularemia

Max Maurin, Bernard Castan, Nathalie Roch

    Emerging Infectious Diseases
    |December 25, 2009
    PubMed
    Abstract

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    Real Time RT-PCR

    Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
    The real-time quantification of the number of amplified products is...