Related Experiment Video
Updated: Jun 17, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
The challenges of the analysis of basic compounds by high performance liquid chromatography: some possible approaches
1Centre for Research in Biomedicine, University of the West of England, Frenchay, Bristol BS16 1QY, UK. david.mccalley@uwe.ac.uk
Abstract:
This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed. Hydrophilic interaction chromatography is considered as an alternative method for the separation of some basic compounds.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Instrumentation
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
High-Performance Liquid Chromatography: Types of Detectors