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Updated: Jun 17, 2026
Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation
Published on: May 17, 2021
Selective collection and detection of leukemia cells on a magnet-quartz crystal microbalance system using
Yuliang Pan1, Manli Guo, Zhou Nie
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Abstract:
A novel method for selective collection and detection of human acute leukemia cells has been proposed using aptamer-conjugated magnetic beads (apt-MBs) and a magnet-quartz crystal microbalance (QCM) system. The sgc8c aptamer-conjugated MBs specifically binding to CCRF-CEM cells were used for target cell extraction from complex matrixes, and the magnet-QCM system was successfully applied for quantitative cell detection, requiring no further labeling of cells. The accumulation of MBs-conjugated CCRF-CEM cells on a quartz crystal gold electrode surface under a magnetic field resulted in decreased resonant frequency. A linear relationship between the frequency shift and cell concentration over the range of 1 x 10(4)-1.5 x 10(5)cells mL(-1) was obtained, with a detection limit of 8 x 10(3)cells mL(-1). The applicability of the method for target cell detection from cell mixture was satisfactory.
