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Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Inflammatory Bowel Disease III: Crohn's Disease01:25

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

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Related Experiment Video

Updated: Jun 17, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
10:31

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Circulating Th17, Th22, and Th1 cells are increased in psoriasis.

Shinji Kagami1, Heather L Rizzo, Jennifer J Lee

  • 1Department of Dermatology, Oregon Health and Science University, Portland, Oregon, USA.

The Journal of Investigative Dermatology
|December 25, 2009
PubMed
Summary

Elevated levels of circulating T helper 17 (Th17), Th22, and Th1 cells are found in psoriasis patients. These inflammatory cells may drive skin and systemic inflammation in psoriasis.

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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

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Last Updated: Jun 17, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
10:31

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Area of Science:

  • Immunology
  • Dermatology

Background:

  • Psoriatic skin lesions contain Th17, Th22, and Th1 cells, implicated in disease pathogenesis.
  • The precise numbers and relative importance of these circulating inflammatory T cells in psoriasis remain unclear.

Purpose of the Study:

  • To quantify circulating Th17, Th22, and Th1 cells in untreated psoriasis patients compared to healthy individuals.
  • To identify optimal cell surface markers for defining these T cell subsets.

Main Methods:

  • Quantification of circulating Th17, Th22, and Th1 cells using 7-color flow cytometry.
  • Analysis of cell surface markers, including CCR6, IL-23R, and CD161.
  • In vitro inhibition of NF-kappaB and STAT3 pathways to assess cytokine production.

Main Results:

  • CCR6 identified as the optimal marker for IL-17A+ cells.
  • Significantly elevated numbers of circulating Th17, Th22, and Th1 cells were observed in psoriasis patients (P<0.001 for Th17).
  • Th17 and Th1 cell levels decreased in patients treated with infliximab.

Conclusions:

  • Elevated circulating Th17, Th22, and Th1 cells contribute to the cutaneous and systemic inflammation in psoriasis.
  • These findings highlight the role of specific T cell subsets in psoriasis pathogenesis and suggest potential therapeutic targets.