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Published on: September 19, 2016
[Primary study on mechanism of baicalin on the Th1/Th2 response in murine model of asthma]
Feng Huang1, Xiao-yun Tong, Hua-ming Deng
1College of Herbal Pharmacy, Jinan University, Guangzhou 510632, China. hftxyy@yahoo.com.cn
Objective:
To study the mechanism of baicalin on the cytokines of Th1/Th2 in murine model of asthma.
Methods:
The murine model of asthma was induced by OVA. Different doses of baicalin were orally administered to the mice respectively. The spleen cells were cultured 3 days for the measurement of IFN-gamma, IL-4, IL-5 and IL-10 by ELISA. After 2 days of culture, the spleen cells were treated with Trizol for extraction of total RNA. The gene expressions of T-bet, GATA-3 and STAT-6 were analyzed by RT-PCR.
Results:
The treatment with baicalin obviously decreased the production of IL-4 and IL-5 and the gene expression of GATA-3, STAT-6, but increased the production of IL-10.
Conclusion:
Baicalin may modulate the Th1/Th2 balance mainly by altering the gene expressions of GATA-3 and STAT-6 in vivo and increasing the production of IL-10.
