[Establishment of mouse model of humoral immune response using rabbit red blood cells as the antigen]

To establish a mouse model of humoral immune response by immunization with rabbit red blood cells (RRBCs).

The mice were immunized with RRBCs and the serum hemolysin level was measured by micro-hemolysis spectrophotometry.

The peak time needed for hemolysin production against RRBCs was 6 days after the immunization, and 20% RRBCs in a total volume of 0.2 ml was optimal for intraperitoneal injection. Hydrocortisone (25 mg/kg) and cyclophosphamide (20 mg/kg) inhibited hemolysin production. Mannatide (4 mg/kg) produced no significant effect on serum hemolysin level in normal mice, but significantly potentiated hemolysin production in immunosuppressed mice induced by cyclophosphamide (20 mg/kg).

Intraperitoneal RRBC injection is feasible for establishing mouse models of humoral immune response.