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Updated: Jun 17, 2026
Mouse Model of Acute to Chronic Kidney Disease Transition Induced by Renal Ischemia/Reperfusion Injury
Published on: February 10, 2026
[Dynamic changes of serum MCP-1 and MIP-2 chemokines after renal transplantation in rats]
1Department of Urology, School of Clinical Medicine, Yangzhou University, Yangzhou 225001, China. yjj_xz@sina.com
Objective:
To investigate the changes in serum levels of chemokine MCP-1 and MIP-2 after renal transplantation in rats and the influence of Cyclosporin A (CsA) on their levels.
Methods:
Three groups of rats, namely untreated group, CsA group and isograft group underwent the renal transplantation, and the serum MCP-1 and MIP-2 levels of the recipients were tested using enzyme-linked immunosorbent assay (ELISA).
Results:
The serum MCP-1 level peaked 6 hours after the operation and also in critical stages of acute rejection. The first peak of MCP-1 was postponed by the application of CsA, which did not affect the peak level (P>0.05). The second peak of MCP-1 did not occur in CsA-treated group. MIP-2 concentration also peaked at 6 h after the operation in all the groups. The second peak of MIP-2, which was lower than the first one (P<0.05), occurred 9 days after the transplantation.
Conclusion:
MCP-1 and MIP-2 in involved not only in the ischemia-reperfusion injury but also in severe acute rejection. CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats.