Construction of an amperometric triglyceride biosensor using PVA membrane bound enzymes

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Enzyme Technology

Background:

• Triglyceride (TG) levels are critical indicators of cardiovascular health.
• Accurate and rapid TG measurement is essential for clinical diagnostics.
• Development of sensitive biosensors is crucial for point-of-care testing.

Purpose of the Study:

• To develop an amperometric triglyceride biosensor utilizing polyvinyl alcohol (PVA) membrane-bound enzymes.
• To optimize the biosensor's performance for TG determination.
• To evaluate the biosensor's accuracy, sensitivity, and stability.

Main Methods:

• Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase, and horseradish peroxidase onto a PVA membrane using glutaraldehyde.
• Amperometric detection of TG at a polarized potential of 0.4 V.
• Optimization of response time, pH, and temperature.

Main Results:

• Optimal sensor performance achieved at pH 7.0 and 25°C with a response time of 2 seconds.
• Linear correlation between current and TG concentration (0.56–2.25 mM) with a detection limit of 0.21 mM.
• High analytical recovery (94.3%) and low coefficients of variation (<5.85% within-batch, <4.13% between-batch).
• Minimal interference from other serum substances, except for slight stimulation by cholesterol.

Conclusions:

• An effective amperometric method for TG determination was developed using an enzyme electrode.
• The biosensor demonstrates high sensitivity, accuracy, and stability for TG measurement.
• This technology holds promise for routine clinical analysis of triglycerides.