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05:34
A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting
Published on: November 20, 2015
Sdc1 negatively modulates carcinoma cell motility and invasion
Tohru Ishikawa1, Randall H Kramer
1Department of Cell and Tissue Biology, University of California San Francisco, 521 Parnassus Avenue, Room C-640, San Francisco, CA 94143-0640, USA.
Experimental Cell Research
|December 29, 2009
Summary
Syndecan-1 (Sdc1) is a novel collagen receptor on squamous cell carcinoma cells that regulates motility and invasion. Its downregulation enhances tumor cell invasiveness and metastasis during cancer progression.
Area of Science:
- Cell Biology
- Cancer Research
- Extracellular Matrix Biology
Background:
- Tumor cells invade collagen-rich extracellular matrix during cancer progression.
- Squamous cell carcinoma cells adhere to collagen I but show limited motility and invasion.
Purpose of the Study:
- Identify collagen receptors involved in tumor cell adhesion and motility.
- Investigate the role of Syndecan-1 (Sdc1) in regulating cell behavior on collagen I.
Main Methods:
- Utilized siRNA-mediated depletion to silence Sdc1 expression in squamous cell carcinoma cells.
- Assessed cell adhesion, spreading, and motility on collagen I substrates.
- Analyzed focal adhesion plaque formation and RhoA/Rac1 activation.
Main Results:
- Syndecan-1 (Sdc1) acts as a collagen I receptor alongside alpha2beta1 integrin.
- Sdc1 depletion reduced adhesion to collagen I but enhanced cell spreading and motility.
- Silencing Sdc1 altered RhoA and Rac1 activation, impacting actin remodeling and motility.
Conclusions:
- Sdc1 is a key regulator of tumor cell motility and invasion on collagen I by modulating RhoA and Rac activity.
- Downregulation of Sdc1 may promote tumor cell invasiveness and metastasis.
- A co-adhesion receptor system involving Sdc1 and integrins governs cell behavior on the extracellular matrix.
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