Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Tooth Anatomy01:21

Tooth Anatomy

The human tooth enables us to eat a variety of foods, speak clearly, and even aid in shaping our faces. Teeth are composed of various elements that work together. Here's a detailed look at the anatomy of a human tooth.
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or grinding food.
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TNAP-mediated hydrolysis of phosphorylated enamel matrix proteins drives local phosphate generation and enamel mineralization.

The Journal of biological chemistry·2026
Same author

Solute carrier family 9 member A5 regulated by TGF-β is necessary for dental enamel formation.

Journal of oral biosciences·2026
Same author

Developmental vulnerability to fluoride toxicity: enamel and clearance differences in adolescent versus mature mice.

Archives of toxicology·2025
Same author

Fibroblast growth factor 2 promotes matrix vesicle-mediated mineralization of human umbilical cord perivascular cells by regulating phosphate metabolism.

Journal of oral biosciences·2025
Same author

Direct and indirect effects of transforming growth factor-beta on osteoclast-mediated bone remodeling using a new in vitro bone matrix model.

JBMR plus·2025
Same author

Citrate Transporter Expression and Localization: The <i>Slc13a5</i><sup>Flag</sup> Mouse Model.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Jun 17, 2026

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Dentin Sialophophoprotein (DSPP) and Dentin.

Yasuo Yamakoshi1

  • 1Department of Biologic and Materials Sciences, University of Michigan Dental Research Lab 1210 Eisenhower Place, Ann Arbor, MI 48108, USA.

Journal of Oral Biosciences
|September 28, 2011
PubMed
Summary

Mutations in dentin sialophosphoprotein (DSPP) cause inherited dental malformations. Research characterizes DSPP-derived proteins, crucial for dentin formation and biomineralization.

Area of Science:

  • Biochemistry
  • Genetics
  • Developmental Biology

Background:

  • Mutations in type I collagen genes cause osteogenesis imperfecta with dentin malformations.
  • Dentin sialophosphoprotein (DSPP) is the most abundant non-collagenous protein in dentin.
  • DSPP mutations lead to inherited dental disorders like dentinogenesis imperfecta and dentin dysplasia.

Purpose of the Study:

  • To characterize the structure and function of DSPP-derived proteins.
  • To understand the role of DSPP in dentin formation and biomineralization.
  • To investigate DSPP cleavage products: dentin sialoprotein (DSP), dentin glycoprotein (DGP), and dentin phosphoprotein (DPP).

Main Methods:

  • Proteomic analysis of DSPP cleavage products.
  • Biochemical characterization of DSP, DGP, and DPP.

More Related Videos

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Related Experiment Videos

Last Updated: Jun 17, 2026

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

  • Investigation of protein modifications and structural properties.

    • Main Results:

    • DSPP is proteolytically cleaved into DSP, DGP, and DPP.
    • DSP is a proteoglycan forming covalent dimers.
    • DGP is a phosphorylated glycoprotein, and DPP is a highly phosphorylated intrinsically disordered protein with genetic polymorphisms.

    Conclusions:

    • DSPP is essential for proper dentin formation and biomineralization.
    • Understanding DSPP-derived proteins provides insights into dentin development.
    • DSPP genetic heterogeneity influences DPP structure and function.