Related Experiment Video
Updated: Jun 17, 2026

Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET
Published on: January 12, 2024
Water-forming NADH oxidase protects Torulopsis glabrata against hyperosmotic stress
1State Key Laboratory of Food Science and School of Biotechnology and Key Technology and Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, People's Republic of China.
Abstract:
A heterologous water-forming NADH oxidase was introduced into Torulopsis glabrata and the effect on cell growth under hyperosmotic conditions was investigated. Expression of the noxE gene from Lactococcus lactis NZ9000 in T. glabrata resulted in a marked decrease in the NADH : NAD+ ratio and higher activities of key enzymes in water-regenerating pathways, leading to an increase in intracellular water content. NaCl-induced reactive oxygen species production was also decreased by the introduction of NADH oxidase, resulting in a significant increase in the growth of T. glabrata under hyperosmotic stress conditions (3824 mOsmol/kg). The results indicated that the osmotolerance of cells can be enhanced by manipulating water-production pathways.
Related Concept Videos
Factors Influencing Microbial Growth: Osmolarity
Responses to Salt Stress
Responses to Drought and Flooding
Tonicity in Plants
Tonicity in Plants
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Tonicity in Animals
