Related Experiment Video
Updated: Jun 17, 2026
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
[Application of immunomagnetic separation to E. coli O157 : H7 detection]
Qian-Qian Tang1, Jian-Ping Wang, Zun-Zhong Ye
1College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China. tangqq252@163.com
In the present paper, the capture efficiencies of four different proportions of E. coli O157 : H7 and immunomagnetic beads (IMB) (1 : 6.34, 1 : 12.69, 1 : 25.38 and 1 : 30.07) and three different proportions of E. coli O157 : H7 and Bacillus subtilis (0.3 mL : 0.1 mL, 0.2 mL : 0.2 mL and 0.1 mL : 0.3 mL) were compared, with the total volume and capacity of IMB remaining constant. It was revealed that the capture efficiency could reach 100% when the proportion of E. coli O157 : H7 and IMB was 1 : 30, meaning that almost all targets E. coli O157 : H7 could be captured at 1 : 30, and that the capture efficiency decreased and then increased with the number of Bacillus subtilis increasing. Moreover, the combination of ATP bioluminescence and IMS was correlated well with conventional culture methods, R2 = 0.9882, and the detection limit was as low as 10(2) CFU x mL(-1).
In the present paper, the capture efficiencies of four different proportions of E. coli O157 : H7 and immunomagnetic beads (IMB) (1 : 6.34, 1 : 12.69, 1 : 25.38 and 1 : 30.07) and three different proportions of E. coli O157 : H7 and Bacillus subtilis (0.3 mL : 0.1 mL, 0.2 mL : 0.2 mL and 0.1 mL : 0.3 mL) were compared, with the total volume and capacity of IMB remaining constant. It was revealed that the capture efficiency could reach 100% when the proportion of E. coli O157 : H7 and IMB was 1 : 30, meaning that almost all targets E. coli O157 : H7 could be captured at 1 : 30, and that the capture efficiency decreased and then increased with the number of Bacillus subtilis increasing. Moreover, the combination of ATP bioluminescence and IMS was correlated well with conventional culture methods, R2 = 0.9882, and the detection limit was as low as 10(2) CFU x mL(-1).
