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Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

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Amelogenin nanoparticles in suspension: deviations from spherical shape and pH-dependent aggregation.

Barbara Aichmayer1, Felicitas B Wiedemann-Bidlack, Christoph Gilow

  • 1Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany. aichmayer@mpikg.mpg.de

Biomacromolecules
|December 30, 2009
PubMed
Summary

Amelogenin proteins self-assemble into oblate nanoparticles in solution, not spheres. This discovery, using SAXS and DLS, is crucial for understanding dental enamel biomineralization.

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Area of Science:

  • Biochemistry
  • Biomaterials Science
  • Dental Research

Background:

  • Amelogenin is known to self-assemble into nanoparticles, often called nanospheres.
  • The precise three-dimensional shape of amelogenin nanoparticles in solution remains largely uncharacterized.

Purpose of the Study:

  • To investigate the three-dimensional shape of recombinant amelogenins (rP172 and rM179) in suspension.
  • To determine the influence of pH on amelogenin nanoparticle shape and aggregation.

Main Methods:

  • Small-Angle X-ray Scattering (SAXS) was employed to analyze nanoparticle structure.
  • Dynamic Light Scattering (DLS) was used to assess particle size and behavior in solution.
  • Experiments were designed to avoid protein contact with support surfaces.

Main Results:

  • Amelogenins form oblate (flattened disc-like) nanoparticles in suspension, not spheres.
  • SAXS data revealed isolated nano-oblates with aspect ratios of 0.45-0.5 at pH > 7.2.
  • Aggregation of these nano-oblates was observed at pH < 7.2.

Conclusions:

  • The oblate shape of amelogenin nanoparticles is a novel finding with implications for enamel formation.
  • The oblate shape may play a key role in forming chain-like structures essential for dental enamel biomineralization.
  • Understanding amelogenin nanoparticle structure provides insights into biomineralization processes.