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Updated: Jun 17, 2026
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Published on: October 18, 2016
Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells
Tamara Brckalo1, Federica Calzetti, Begoña Pérez-Cabezas
1Immunology Unit, Department of Experimental and Health Sciences, University Pompeu Fabra, Barcelona, Spain.
Abstract:
The CD300e surface molecule, originally termed immune receptor expressed by myeloid cells (IREM)-2, was reported to associate with the DNAX-activating protein (DAP) 12 adaptor in co-transfected cells, and is capable of signaling. In the present report, we investigated in detail the function of CD300e in monocytes and myeloid DC (mDC) freshly isolated from peripheral blood of normal blood donors. Upon engagement by an agonistic mAb, CD300e triggered an intracellular calcium mobilization and superoxide anion O(2) (-) production in monocytes. Activation via CD300e provided survival signals that prevented monocyte and mDC apoptosis, triggered the production of pro-inflammatory cytokines and upregulated the expression of cell surface co-stimulatory molecules in both cell types. Moreover, CD300e activation of mDC enhanced the alloreactive response of naive T cells. Overall, our data formally support the notion that CD300e functions as an activating receptor capable of regulating the innate immune response in myeloid cells.
Insights
The CD300e molecule, also known as immune receptor expressed by myeloid cells-2 (IREM-2), acts as an activating receptor on myeloid cells. It enhances innate immune responses by promoting cytokine production and T cell activation.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- CD300e, previously known as immune receptor expressed by myeloid cells-2 (IREM-2), is a surface molecule expressed on myeloid cells.
- Previous studies suggested its association with the DNAX-activating protein (DAP) 12 adaptor and signaling capability.
Purpose of the Study:
- To investigate the function of CD300e in primary human monocytes and myeloid dendritic cells (mDCs).
- To elucidate the role of CD300e in regulating innate immune responses.
Main Methods:
- Isolation of monocytes and mDCs from peripheral blood of healthy donors.
- Stimulation with an agonistic monoclonal antibody (mAb) against CD300e.
- Analysis of intracellular calcium mobilization, superoxide anion production, cytokine release, co-stimulatory molecule expression, and T cell alloreactive responses.
Main Results:
- CD300e engagement induced calcium mobilization and superoxide anion production in monocytes.
- Activation of CD300e conferred survival signals, preventing apoptosis in monocytes and mDCs.
- CD300e triggered pro-inflammatory cytokine production and upregulated co-stimulatory molecules on both cell types.
- CD300e activation of mDCs enhanced the alloreactive response of naive T cells.
Conclusions:
- CD300e functions as a potent activating receptor on myeloid cells.
- CD300e plays a significant role in regulating innate immunity, influencing cytokine production, cell survival, and T cell responses.
