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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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Related Experiment Video

Updated: Jun 17, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Multilocus sequence typing of Clostridium difficile.

David Griffiths1, Warren Fawley, Melina Kachrimanidou

  • 1Nuffield Department of Clinical Medicine, Oxford University, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom.

Journal of Clinical Microbiology
|January 1, 2010
PubMed
Summary
This summary is machine-generated.

A new multilocus sequence typing (MLST) method accurately genotypes Clostridium difficile, enabling rapid identification and improved infection control. This robust scheme aids in tracking bacterial strains and understanding their evolution.

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Published on: October 30, 2016

Related Experiment Videos

Last Updated: Jun 17, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

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Published on: December 10, 2016

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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Clostridium difficile infections pose a significant healthcare challenge.
  • Accurate and rapid genotyping is crucial for effective infection control and patient management.

Purpose of the Study:

  • To develop and validate a robust, high-throughput multilocus sequence typing (MLST) scheme for Clostridium difficile.
  • To establish a web-accessible database for sharing Clostridium difficile MLST data.
  • To assess the utility of MLST for direct genotyping from clinical samples.

Main Methods:

  • Developed and validated a multilocus sequence typing (MLST) scheme using 50 reference isolates and 102 clinical samples.
  • Compared MLST with PCR ribotyping for discriminatory ability.
  • Constructed phylogenetic trees from concatenated MLST sequences.
  • Tested direct genotyping from total stool DNA extracts.

Main Results:

  • The MLST scheme identified 40 sequence types (STs) across 49 PCR ribotypes.
  • MLST and PCR ribotyping showed similar discriminatory abilities (0.90 vs. 0.92).
  • Phylogenetic analysis revealed four distinct phylogenetic groups of STs.
  • Direct genotyping from stool DNA without culture was successful.

Conclusions:

  • The developed MLST scheme is a robust and discriminatory tool for Clostridium difficile genotyping.
  • The web database facilitates data sharing and comparison among laboratories.
  • Direct MLST from stool samples offers a rapid genotyping method beneficial for patient care and infection control.