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Updated: Jun 17, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Collective fluorescence blinking in linear J-aggregates assisted by long-distance exciton migration
Hongzhen Lin1, Rafael Camacho, Yuxi Tian
1Chemical Physics, Lund University, Box 124, 22100, Lund, Sweden.
Abstract:
Fluorescence blinking corresponding to collective quenching of up to 100 dye monomers is reported for individual J-aggregates of a perylene bisimide (PBI) dye. This implies an exciton diffusion length up to 70 nm in these one-dimensional assemblies. The number of quenched monomers was directly measured by comparing the fluorescence brightness of the J-aggregates with that of noncoupled PBI molecules. This brightness analysis technique is useful for unraveling photophysical parameters of any individual fluorescent nanosystem.
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