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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: Jun 17, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Alternative splicing regulatory network reconstruction from exon array data.

Kun Qu1, Anastasia M Yesnik, Peter J Ortoleva

  • 1Center for Cell and Virus Theory, Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. kqu@indiana.edu

Journal of Theoretical Biology
|January 2, 2010
PubMed
Summary

Alternative splicing (AS) regulation is crucial for human cells but can lead to disease. This study presents a method to reconstruct the human alternative splicing regulatory network (SRN) for diagnostic and therapeutic insights.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Pre-mRNA alternative splicing (AS) generates diverse mRNA and protein isoforms, essential for cellular function.
  • Dysregulation of AS is implicated in various human diseases, including cancer.
  • AS is orchestrated by splicing regulators like SR proteins, hnRNPs, and snRNPs, forming a complex regulatory network (SRN).

Purpose of the Study:

  • To develop a methodology for automating the genome-wide reconstruction of the human alternative splicing regulatory network (SRN).
  • To facilitate the design of diagnostic and therapeutic strategies by understanding defects in the SRN.

Main Methods:

  • Genome-wide SRN reconstruction based on correlation analysis of human exon expression and conventional gene expression microarray data.
  • Utilized an experimentally verified training set of AS and transcriptional regulatory interactions.
  • Developed automated software (AutoNet) for SRN reconstruction.

Main Results:

  • A genome-wide SRN was successfully constructed for normal human cells.
  • An assessment of the reliability of each predicted interaction within the SRN was provided.
  • The reconstructed human SRN is publicly available via a web portal.

Conclusions:

  • Automated reconstruction of the human SRN is feasible using integrated expression data and interaction sets.
  • The developed methodology and software (AutoNet) enable efficient SRN analysis.
  • The publicly available SRN data can aid in disease research and the development of novel therapeutic approaches.