Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Focal Impact Model of Traumatic Brain Injury in <i>Xenopus</i> Tadpoles Reveals Behavioral Alterations, Neuroinflammation, and an Astroglial Response.

International journal of molecular sciences·2022

Same author

miR-199 plays both positive and negative regulatory roles in Xenopus eye development.

Genesis (New York, N.Y. : 2000)·2020

Same author

Commentary and tribute to Antone Jacobson: The pioneer of morphodynamics.

Developmental biology·2019

Same author

Phosphaplatin Anti-tumor Effect Enhanced by Liposomes Partly <i>via</i> an Up-regulation of PEDF in Breast Cancer.

Anticancer research·2018

Same author

Using Xenopus to understand human disease and developmental disorders.

Genesis (New York, N.Y. : 2000)·2017

Same author

Data on microRNAs and microRNA-targeted mRNAs in <i>Xenopus</i> ectoderm.

Data in brief·2016

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

FGFR3 expression in Xenopus laevis.

Amanda Popielski Pope¹, Chen Liu, Amy K Sater

¹Department of Biology, Ithaca College, Ithaca, NY 14850, USA.

Gene Expression Patterns : GEP

|January 2, 2010

Summary

This summary is machine-generated.

Fibroblast Growth Factor Receptor 3 (FGFR3) mRNA is expressed in early Xenopus development, particularly in neural and mesodermal tissues. Its conserved expression pattern suggests important roles in vertebrate embryonic development.

More Related Videos

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Published on: May 23, 2010

Related Experiment Videos

Last Updated: Jun 17, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Published on: May 23, 2010

Area of Science:

Developmental Biology
Molecular Biology
Genetics

Background:

Fibroblast Growth Factors (FGFs) and their receptors play crucial roles in embryonic development.
FGFR3 is a key receptor in the FGF signaling pathway, but its expression patterns in early vertebrate development are not fully elucidated.

Purpose of the Study:

To investigate the spatiotemporal expression pattern of Fibroblast Growth Factor Receptor 3 (FGFR3) mRNA during early development of Xenopus laevis.
To compare FGFR3 expression with known FGF expression patterns to infer potential signaling interactions.

Main Methods:

Reverse Transcription Polymerase Chain Reaction (RT-PCR) was used to detect FGFR3 mRNA.
Whole-mount in situ hybridization was employed to visualize the spatial distribution of FGFR3 mRNA in developing embryos.

Main Results:

FGFR3 mRNA was detected starting in the gastrula stage of Xenopus development.
Regionalized expression was observed by the neural plate stage and persisted throughout embryogenesis.
Strong FGFR3 expression was identified in developing neural structures (forebrain, hindbrain, eyes) and lateral mesoderm.

Conclusions:

The expression pattern of FGFR3 in Xenopus laevis is highly localized and dynamic during early development.
The observed expression domains suggest potential roles for FGF-FGFR3 signaling in neural and mesodermal development.
The expression pattern of FGFR3 in Xenopus is conserved across vertebrate embryos, highlighting its fundamental importance.