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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Biotechnological applications of microbial proteomes.
Mee-Jung Han1, Sang Yup Lee, Seung-Tae Koh
1Department of Chemical and Biomolecular Engineering, Dongyang University, # 1 Gyochon-dong, Punggi-eup, Yeongju, Gyeongbuk 750-711, Republic of Korea. mjhan75@dyu.ac.kr
Journal of Biotechnology
|January 5, 2010
Summary
Proteomics enables the discovery of novel biotechnological targets, including promoters and fusion partners. This technology aids in understanding physiological networks and optimizing bioprocesses for scientific and industrial applications.
Area of Science:
- Biotechnology
- Proteomics
- Biochemistry
Background:
- Proteomic technologies generate large-scale proteome databases.
- These databases elucidate metabolic, signaling, and regulatory network dynamics.
- Understanding physiological changes is crucial in biological research.
Purpose of the Study:
- Describe proteomic approaches for discovering biotechnological targets.
- Identify specific targets like promoters, chaperones, and fusion partners.
- Discuss applications in bioprocess design and optimization.
Main Methods:
- Review of proteomic technologies and their applications.
- Analysis of large-scale proteome databases.
- Identification of protein targets for biotechnology.
Main Results:
- Proteomics facilitates the identification of proteins with biotechnological potential.
- Key targets include promoters, chaperones, soluble fusion partners, anchoring motifs, and excretion fusion partners.
- Proteomic data offers insights into protein functions and physiological actions.
Conclusions:
- Proteomic approaches are valuable for discovering targets in biotechnology.
- Proteomics aids in designing future bioprocesses and optimizing existing ones.
- The application of proteomic information holds significant scientific and practical value.
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