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Second-generation bioethanol from eucalypt sulphite spent liquor.

Ana M R B Xavier¹, Mariana F Correia, Susana R Pereira

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|January 5, 2010

Summary

Bioethanol production from Eucalyptus globulus spent liquor requires removal of inhibitory compounds. Complete purification of the liquor significantly improved fermentation yields using Pichia stipitis yeast.

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Area of Science:

Biotechnology
Biochemical Engineering
Renewable Energy

Background:

Spent liquor from acidic sulphite pulping of Eucalyptus globulus (HSSL) contains fermentable sugars from degraded hemicelluloses.
This lignocellulosic byproduct presents a potential feedstock for bioethanol production.

Purpose of the Study:

To investigate the feasibility of producing bioethanol from HSSL using Pichia stipitis fermentation.
To identify and overcome inhibitory factors affecting ethanol yield in HSSL.

Main Methods:

Fermentation experiments using Pichia stipitis with varying concentrations of HSSL.
Biological and chemical treatments (ion-exchange resins) to remove inhibitory compounds like acetic acid and polyphenolics.
Analysis of ethanol yield, productivity, and cell growth rate.

Main Results:

Increasing HSSL concentration negatively impacted ethanol yield, with yields dropping from 0.15 g/g to 0.08 g/g sugar consumed at 20% and 60% HSSL, respectively.
Biological removal of acetic acid showed partial improvement, but complete removal of acetic acid and polyphenolics via ion-exchange resins was crucial for high yields.
Optimized conditions achieved a fair ethanol yield (Yp/s = 0.49 g/g) and productivity with P. stipitis.

Conclusions:

HSSL can be utilized for bioethanol production, but pretreatment to remove inhibitory substances is essential.
Effective purification strategies are key to enhancing the efficiency of Pichia stipitis fermentation for bioethanol from lignocellulosic materials.
This study demonstrates a viable pathway for valorizing pulp mill side streams into biofuels.