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Related Experiment Video

Updated: Jun 17, 2026

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice
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Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice

Published on: February 12, 2018

[Antiaging research using klotho mice].

Hiroshi Manya1, Keiko Akasaka-Manya, Tamao Endo

  • 1Molecular Glycobiology, Tokyo Metropolitan Institute of Gerontology, Foundation for Research on Aging and Promotion of Human Welfare, Tokyo, Japan. manya@tmig.or.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|January 5, 2010
PubMed
Summary

The alpha-klotho gene mutation in mice causes aging phenotypes by affecting proteolysis. Understanding klotho protein

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Area of Science:

  • Gerontology
  • Molecular Biology
  • Genetics

Background:

  • The klotho mouse model exhibits phenotypes mirroring human aging due to a mutation in the alpha-klotho gene.
  • Alpha-klotho protein, primarily in the kidney and brain, plays a crucial role in regulating aging processes.
  • Defects in alpha-klotho gene expression lead to age-associated disorders, including arteriosclerosis and osteoporosis.

Purpose of the Study:

  • To elucidate the mechanism by which alpha-klotho protein suppresses aging phenomena.
  • To investigate the role of proteolysis in age-related disorders using the klotho mouse model.
  • To understand the molecular mechanisms underlying human aging through klotho mouse pathophysiology.

Main Methods:

  • Analysis of the pathophysiology of klotho mice.
  • Investigating the aberrant activation of mu-calpain caused by alpha-klotho mutation.
  • Comparing klotho mice with normal aged mice to identify age-related molecular changes.

Main Results:

  • Alpha-klotho mutation leads to aberrant mu-calpain activation and cytoskeletal degradation.
  • Similar proteolysis phenomena are observed in normal aged mice, suggesting a conserved aging mechanism.
  • Klotho protein appears to suppress age-related tissue deterioration processes.

Conclusions:

  • The alpha-klotho gene product plays a critical role in suppressing aging.
  • Proteolysis, particularly mu-calpain activity, is implicated in age-related disorders.
  • Further research on klotho protein function can provide insights into aging and age-related diseases.

Related Experiment Videos

Last Updated: Jun 17, 2026

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice
06:49

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice

Published on: February 12, 2018