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Related Experiment Video

Updated: Jun 17, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

[Anti-aging research using Mn-SOD conditional knockout mice].

Takahiko Shimizu1, Takuji Shirasawa

  • 1Molecular Gerontology, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan. shimizut@tmig.or.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|January 5, 2010
PubMed
Summary

Manganese superoxide dismutase (Mn-SOD) deficiency causes severe heart and muscle issues in adult mice, but not liver problems. These new tissue-specific knockout mice are valuable tools for studying oxidative stress diseases.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Context:

  • Mitochondrial enzyme Manganese superoxide dismutase (Mn-SOD) detoxifies superoxide radicals.
  • Systemic Mn-SOD deficiency in mice leads to neonatal lethality, preventing adult tissue studies.
  • A need exists for adult mouse models to investigate oxidative stress pathologies.

Purpose:

  • To generate tissue-specific Mn-SOD conditional knockout mouse models for studying adult oxidative stress.
  • To investigate the role of Mn-SOD in liver, heart, and skeletal muscle pathologies.

Summary:

  • Liver-specific Mn-SOD knockout mice showed no significant abnormalities, suggesting limited physiological importance in the liver.
  • Heart/muscle-specific Mn-SOD knockout mice developed dilated cardiomyopathy and mitochondrial respiration defects.

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Last Updated: Jun 17, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
08:12

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

Published on: November 1, 2011

  • Skeletal muscle-specific Mn-SOD knockout mice exhibited physical disturbances linked to impaired ATP metabolism.

    • Impact:

    • Mitochondrial superoxide is crucial for heart and skeletal muscle pathologies, but not liver disease.
    • Established tissue-specific Mn-SOD knockout mice serve as essential tools for oxidative stress research.
    • Provides insights into the tissue-specific roles of Mn-SOD in disease pathogenesis.