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Modified method of mammalian cell synchronization improves yield and degree of synchronization
1Physiology Department, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Experimental Cell Research
|April 1, 1991
Abstract:
A modified method to synchronize CHO and HeLa cells is developed based upon a combined shaking-off and chemical blockage. This method has effectively blocked quiescent cells, which is the main obstacle of high degree synchronization. Flow-cytometry data show the improvement on the degree of synchronization and yield compared to two previously used methods.