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Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma.
M M Wanjari1, A W There, M R Tajne
1Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur-440 033, India.
Indian Journal of Pharmaceutical Sciences
|January 5, 2010
Summary
A new high-performance liquid chromatography method accurately measures metformin in rat plasma. This validated assay is suitable for pharmacokinetic studies of oral metformin in rats.
Area of Science:
- Analytical Chemistry
- Pharmacokinetics
Background:
- Metformin is a widely used antidiabetic drug.
- Accurate quantification of metformin in biological matrices is crucial for pharmacokinetic studies.
Purpose of the Study:
- To develop and validate a simple, rapid, and reliable reverse-phase high-performance liquid chromatographic (RP-HPLC) method for determining metformin concentration in rat plasma.
Main Methods:
- Utilized a C(18) column with a mobile phase of ammonium acetate and acetonitrile.
- Employed ultraviolet detection at 236 nm for quantification.
- Acetonitrile served for simultaneous deproteinization and extraction of metformin from plasma samples.
Main Results:
- The assay demonstrated linearity over a concentration range of 0.33-16.6 µg/ml (r² = 0.994).
- The method exhibited good precision (% CV < 15%) and accuracy.
- The retention time for metformin was observed at 4.7 minutes.
Conclusions:
- The developed RP-HPLC method is simple, precise, accurate, and suitable for pharmacokinetic investigations of orally administered metformin in rats.
- This validated method can aid in understanding metformin's absorption, distribution, metabolism, and excretion in preclinical models.