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Updated: Jun 17, 2026

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
09:00

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles

Published on: March 18, 2015

Inhibition of transcription by platinum antitumor compounds

Ryan C Todd1, Stephen J Lippard

  • 1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Metallomics : Integrated Biometal Science
|January 5, 2010
PubMed

Insights

Platinum anticancer drugs like cisplatin induce apoptosis by damaging DNA, inhibiting transcription. Understanding these platinum-DNA adducts

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Pharmacology

Background:

  • Cisplatin, carboplatin, and oxaliplatin are FDA-approved platinum-based anticancer drugs.
  • These drugs function by binding to nuclear DNA, forming adducts.
  • These platinum-DNA adducts trigger cellular responses, including transcription inhibition.

Purpose of the Study:

  • To review structural investigations of platinum-DNA adducts and their effects on DNA geometry.
  • To detail research on transcription inhibition by platinum-DNA adducts.
  • To mechanistically analyze how DNA structural distortions from platinum damage inhibit RNA synthesis.

Main Methods:

  • Review of structural investigations of platinum-DNA adducts.
  • Analysis of research on transcription inhibition by platinum-DNA adducts.
  • Mechanistic analysis of DNA damage-induced RNA synthesis inhibition.

Main Results:

  • Detailed review of Pt-DNA adduct structures and their impact on global DNA geometry.
  • Research findings demonstrating transcription inhibition by Pt-DNA adducts.
  • Mechanistic insights into how DNA distortions caused by platinum drugs impede RNA synthesis.

Conclusions:

  • Understanding the molecular mechanisms of platinum-based anticancer agents is crucial.
  • Elucidating the effects of Pt-DNA adducts on DNA structure and transcription is key.
  • This knowledge will aid in the development of novel platinum-based anticancer drugs.

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