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Na(+)-dependent succinate uptake in Corynebacterium glutamicum.

H Ebbighausen1, B Weil, R Krämer

  • 1Institut für Biotechnologie 1, Forschungszentrum Jülich GmbH, Jülich, F.R.G.

FEMS Microbiology Letters
|January 1, 1991
PubMed
Summary

Corynebacterium glutamicum efficiently transports succinate using a specific carrier. This process is sodium-dependent, indicating a secondary active transport mechanism.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • Corynebacterium glutamicum is a key industrial microorganism.
  • Understanding nutrient uptake is crucial for metabolic engineering.

Purpose of the Study:

  • To elucidate the mechanism of succinate uptake in Corynebacterium glutamicum.
  • To characterize the kinetic and energetic properties of succinate transport.

Main Methods:

  • Cellular uptake assays with washed Corynebacterium glutamicum cells.
  • Kinetic analysis to determine Km and Vmax values.
  • Inhibition studies using fumarate and oxaloacetate.
  • Determination of activation energy and pH optimum.
  • Investigation of the role of Na+ ions and membrane potential.

Main Results:

  • Succinate uptake exhibits Michaelis-Menten kinetics with an apparent Km of ~150 µM and Vmax of 4-7 nmol/(mg dry weight)/min.
  • Uptake is competitively inhibited by fumarate and oxaloacetate.
  • The activation energy for succinate transport is 50 kJ/mol.
  • Transport activity is dependent on extracellular Na+ and membrane potential, with a pH optimum around 8.5.

Conclusions:

  • Succinate is transported into Corynebacterium glutamicum via a specific carrier-mediated system.
  • The transport mechanism is secondary active, driven by a Na+ gradient.
  • These findings provide insights into the metabolic capabilities of C. glutamicum.

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