Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Vaccine Production01:23

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SpyCEP dismantles neutrophil immunity via disorder-driven chemokine remodeling and GAG targeting.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Comparative evaluation of performance and precision of multiplex immunoassays measuring human IgG to <i>Streptococcus pyogenes</i> vaccine antigens.

Frontiers in immunology·2026
Same author

An mRNA-based FimH nanoparticle vaccine against uropathogenic <i>Escherichia coli</i> is highly immunogenic in rodents.

Frontiers in immunology·2025
Same author

Publisher Correction: Early-life serological profiles and the development of natural protective humoral immunity to Streptococcus pyogenes in a high-burden setting.

Nature medicine·2025
Same author

Pulse characterization at the single-photon level through chronocyclic <i>Q</i>-function measurements.

Optics express·2025
Same author

Early-life serological profiles and the development of natural protective humoral immunity to Streptococcus pyogenes in a high-burden setting.

Nature medicine·2025

Related Experiment Video

Updated: Jun 17, 2026

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
05:39

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

Published on: October 30, 2018

Genome-based vaccine development: a short cut for the future.

Danilo Gomes Moriel1, Maria Scarselli, Laura Serino

  • 1Novartis Vaccines, Via Fiorentina 1, 53100, Siena, Italy.

Advances in Experimental Medicine and Biology
|January 5, 2010
PubMed
Summary

Reverse vaccinology utilizes microbial genome data to create new vaccines, overcoming limitations of traditional methods. This approach has led to novel vaccines for diseases like Neisseria meningitidis B.

More Related Videos

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

Published on: October 18, 2022

Related Experiment Videos

Last Updated: Jun 17, 2026

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
05:39

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

Published on: October 30, 2018

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

Published on: October 18, 2022

Area of Science:

  • Microbiology
  • Immunology
  • Vaccinology

Background:

  • Bacterial infectious diseases are a leading global cause of mortality and disability.
  • Conventional vaccinology has limitations in developing effective vaccines for numerous pathogens.
  • The advent of microbial genome sequencing opened new avenues for vaccine development.

Purpose of the Study:

  • To introduce and explain the concept and evolution of reverse vaccinology.
  • To highlight the advantages of reverse vaccinology over conventional approaches.
  • To showcase the success of reverse vaccinology in developing vaccines against challenging bacterial pathogens.

Main Methods:

  • Genomic data analysis to identify potential vaccine candidates.
  • Bioinformatic tools to predict surface-exposed antigens.
  • In silico screening and experimental validation of immunogenic targets.

Main Results:

  • Reverse vaccinology has enabled the development of novel vaccines against challenging pathogens.
  • Successful examples include vaccines against Neisseria meningitidis serogroup B and Streptococcus agalactiae.
  • This approach enhances understanding of host-pathogen interactions for improved vaccine design.

Conclusions:

  • Reverse vaccinology represents a paradigm shift in vaccine development.
  • Leveraging genomic information is crucial for overcoming traditional vaccinology challenges.
  • Continued research in reverse vaccinology holds promise for combating emerging infectious diseases.