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The peripherin gene maps to mouse chromosome 15.
J W Pendleton1, S M Violette, L W Hunihan
1Department of Biology, Yale University, New Haven, Connecticut 06511.
Genomics
|February 1, 1991
Summary
Researchers mapped the mouse peripherin gene (Prph) to chromosome 15 using Southern analysis. This finding aids understanding of intermediate filament gene evolution and neuronal cytoskeleton function.
Area of Science:
- Genetics
- Neuroscience
- Molecular Biology
Background:
- Peripherin is a type III intermediate filament protein.
- It is expressed in both the peripheral and central nervous systems.
- Its precise function remains under investigation, but it is likely involved in the neuronal cytoskeleton.
Purpose of the Study:
- To map the location of the mouse peripherin gene (Prph).
- To contribute to the understanding of intermediate filament gene organization and evolution.
Main Methods:
- Southern blot analysis was performed.
- A panel of Chinese hamster/mouse somatic cell hybrids was utilized.
- A rat peripherin cDNA probe was employed for hybridization.
Main Results:
- The mouse peripherin gene (Prph) was successfully mapped to chromosome 15.
- This mapping was achieved through the analysis of somatic cell hybrids.
Conclusions:
- The chromosomal location of the mouse peripherin gene has been identified.
- This provides a basis for further studies into the gene family's evolution and the protein's function in the neuronal cytoskeleton.