Marginal seal stability of one bottle adhesives in Class V vs. Class I cavities

The aim of this study was to test the influence of two different cavity configurations on marginal stability of recent one bottle "etch & rinse" and "self-etch" adhesives in Class V vs. Class I cavities, before and after thermo-mechanical loading under simulation of dentinal fluid. Forty human upper molars were selected and assigned to five experimental groups. Intrapulpal pressure was maintained during cavity preparation, restoration placement, finishing and stressing. Standardized Class I and V-Shaped Class V cavities were prepared on each tooth. Half of the margins of Class V cavities were located in enamel and half in dentin. All cavities were restored with different adhesives systems and a nano-hybrid composite. Materials were light-cured using a LED unit. Restored teeth were loaded in a computer-controlled chewing machine with 1.2 million mechanical occlusal cycles simultaneously with 3,000 thermal cycles (5-50-5°C). Impressions were made with polyvinylsiloxane of each restoration before and after loading. Gold-coated epoxy replicas were prepared for SEM examination at ×200 magnification. Significant differences between materials were found both before and after loading (Kruskal-Wallis, Bonferroni, p < 0.05). Significant differences were also found between Class I and V restorations (Wilcoxon Matched-Pairs Signed-Rank Test, p < 0.05). Even before thermo-mechanical loading, none of the groups had 100% continuous margin. Marginal seal stability of recent one bottle "etch & rinse" and "self-etch" adhesives are significantly different and susceptible to cavity configuration.