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Updated: Jun 17, 2026

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
05:31

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Published on: December 21, 2014

Isolation of amniotic epithelial stem cells.

Toshio Miki1, Fabio Marongiu, Kenneth Dorko

  • 1University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Current Protocols in Stem Cell Biology
|January 6, 2010
PubMed
Summary

Researchers developed a simple protocol to isolate amniotic epithelial cells from the placenta. This method enriches for stem cells positive for SSEA-4, offering potential for regenerative medicine applications.

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Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Placental tissue engineering

Background:

  • Placental tissues contain cells with phenotypic plasticity, making them valuable for regenerative medicine.
  • Existing isolation procedures for placental stem cells vary.
  • Selective isolation of specific placental cell types is crucial for therapeutic applications.

Purpose of the Study:

  • To describe a detailed and simple protocol for the selective isolation of amniotic epithelial cells from human term placenta.
  • To achieve isolation without disturbing the underlying mesenchymal layer.
  • To introduce a density separation technique for enriching SSEA-4 positive cells.

Main Methods:

  • Selective isolation of amniotic epithelial cells from human term placenta.

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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

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Last Updated: Jun 17, 2026

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
05:31

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Published on: December 21, 2014

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
10:00

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

  • Protocol designed to avoid disruption of the mesenchymal layer.
  • Density separation technique for SSEA-4 positive cell enrichment.

    • Main Results:

    • A detailed and simple protocol for selective isolation of amniotic epithelial cells was established.
    • The method successfully isolated cells without disturbing the mesenchymal layer.
    • A density separation technique effectively enriched for SSEA-4 positive cells.

    Conclusions:

    • Amniotic epithelial cells can be selectively isolated using a straightforward protocol.
    • The described method provides a reliable source of placental stem cells for regenerative medicine.
    • Enrichment for SSEA-4 positive cells enhances the potential therapeutic utility of isolated cells.