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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Optical nanoparticle sensors for quantitative intracellular imaging.

Yong-Eun Koo Lee¹, Raoul Kopelman¹

¹University of Michigan, Ann Arbor, MI, USA.

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology

|January 6, 2010

Summary

Nanoparticle (NP) platforms offer promising tools for intracellular biosensors, enabling real-time, interference-free measurements. This review details NP-based optical sensors and their applications, highlighting advancements toward single-molecule detection within living cells.

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Area of Science:

Biotechnology
Nanotechnology
Cell Biology

Background:

Real-time, interference-free in vivo intracellular measurements are crucial for biological studies.
Nanoparticle (NP) platforms are ideal for developing intracellular biosensors due to their small size and engineerability.

Purpose of the Study:

To review the development and intracellular applications of nanoparticle-based optical sensors.
To classify NP-based sensor designs and discuss their mechanisms.

Main Methods:

Classification of NP-based optical sensors into two types based on receptor and transducer components.
Review of existing literature on NP-based sensor designs and their performance.

Main Results:

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Last Updated: Jun 17, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

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Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

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Type 1 sensors integrate receptor and transducer, functioning like molecular probes.

Type 2 sensors utilize separate receptor and transducer components, often requiring specific NPs for enhanced optical signal or selectivity.

NP-based sensors show potential for synergistic increases in optical signal and selectivity.

Conclusions:

Nanoparticle-based optical sensors are advancing rapidly for intracellular studies.
Future NP systems will enable single-molecule level sensing, simultaneous multi-analyte detection, and measurement of physical parameters within living cells.