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Microbiota of the Stomach and Small Intestine01:27

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Related Experiment Video

Updated: Jun 17, 2026

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
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Published on: April 9, 2019

Helicobacter species in the human colon.

J I Keenan1, C R Beaugie, B Jasmann

  • 1Department of Surgery, University of Otago, Christchurch, New Zealand. jacqui.keenan@otago.ac.nz

Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland
|January 6, 2010
PubMed
Summary

Helicobacter species DNA was found in a significant portion of patients undergoing colonoscopy, but no link to colon disease was identified. Serological tests for Helicobacter pylori showed discrepancies with molecular findings, indicating limitations in detection methods.

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Area of Science:

  • Gastroenterology
  • Microbiology
  • Molecular Diagnostics

Background:

  • Helicobacter species are known pathogens, primarily associated with gastric infections.
  • The presence and role of Helicobacter species in the colon remain less understood.
  • Colonoscopy is a key diagnostic tool for various gastrointestinal conditions.

Purpose of the Study:

  • To investigate the prevalence, frequency, and colonization patterns of Helicobacter species within the colon.
  • To assess the association between Helicobacter species and colon disease.
  • To compare molecular and serological methods for detecting Helicobacter species in the colon.

Main Methods:

  • Colonic biopsies were collected from 100 patients undergoing colonoscopy for non-specific gastrointestinal issues.
  • Group-specific PCR was used to detect Helicobacteraceae family DNA in biopsy samples.
  • Serum IgG reactivity to Helicobacter pylori was analyzed.

Main Results:

  • Helicobacter species DNA was detected in 35% of patients, with Helicobacter pylori identified in 22%.
  • Fifteen patients showed a positive serum IgG response to H. pylori, which did not always correlate with molecular detection in biopsies.
  • No significant association was found between Helicobacter pylori infection and specific colon diseases.

Conclusions:

  • Helicobacter infection is present in a notable number of patients undergoing colonoscopy, but not linked to colon disease.
  • Discrepancies between molecular and serological tests highlight the need for improved diagnostic strategies.
  • Future research on colonic Helicobacter species should utilize a combination of detection methods, not relying solely on serology.