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Published on: June 3, 2020
Ramipril enhances the endogenous hydrogen sulfide tissue concentration in mouse heart and brain
Jerzy Wiliński1, Eugeniusz Somogyi, Marta Góralska
1I Klinika Kardiologii i Nadciśnienia Tetniczego, Collegium Medicum Uniwersytetu Jagiellońskiego, Kraków, Poland. putamen@interia.pl
Background:
Hydrogen sulfide (H2S) participates in regulation of different physiological and pathological processes of cardiovascular system and nervous system. The effect of ramipril - tissue specific angiotensin-converting enzyme inhibitor (ACEI) - exceeds renin-angiotensin aldosterone system (RAAS) blockade. The influence of ACEI on the metabolism of H2S remains obscure. The aim of the study is to assess the impact of ramipril administration on the tissue concentration of H2S in mouse heart and brain.
Method:
Thirty mice (CBA) of both sexes were given intraperitoneal injections of ramipril solution - 0.125 mg (5 mg/kg - group D1) and 0.25 mg (10 mg/kg - group D2) for 5 consecutive days at the same time of the day (10:30 am). Control group received physiological saline in portions of the same volume - 0.2 ml. The measurements of the concentration of H2S were performed using spectrophotometric modified method of Siegel.
Results:
There has been a significance rise in the tissue concentration of H2S [microg/g] in the heart in group D1 (4.91 +/- 0.07 vs 5.54 +/- 0.12; p = 0.005) and group D2 (4.91 +/- 0.07 vs 5.94 +/- 0.08; p < 0.001) and in the brain in group D1 (0.61 +/- 0.03 vs 0.69 +/- 0.02; p = 0.02) and non-significant increase in the brain D2 group (0.61 +/- 0.03 vs 0.65 +/- 0.03; p = 0.16).
Conclusions:
Ramipril enhances the concentration of endogenous H2S in mouse heart and brain.
