Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytomegalovirus Disease01:27

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Application of acupuncture and moxibustion for keeping shape.

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan·1999
Same author

Two-effective-source method for the calculation of in-air output at various source-to-detector distances in wedged fields.

Medical physics·1999
Same author

Follow up of women with simple ovarian cysts detected by transvaginal sonography in the Tokyo metropolitan area.

British journal of obstetrics and gynaecology·1999
Same author

(R)-3,3,3-Trifluoro-2-hydroxy-2-methylpropionamides are orally active inhibitors of pyruvate dehydrogenase kinase.

Journal of medicinal chemistry·1999
Same author

Expression analysis of multidrug resistance associated genes in neuroblastomas.

Oncology reports·1999
Same author

A random survey of the Cryptosporidium parvum genome.

Infection and immunity·1999

Related Experiment Video

Updated: Jun 17, 2026

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
07:35

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Published on: December 24, 2016

Hepatitis C virus modulates human monocyte-derived dendritic cells.

E A Eksioglu1, J R Bess, H Zhu

  • 1Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL 32610-0275, USA.

Journal of Viral Hepatitis
|January 7, 2010
PubMed
Summary
This summary is machine-generated.

Hepatitis C virus (HCV) infection impairs dendritic cell (DC) maturation and suppresses their type 1 interferon response. This suggests a viral mechanism for evading host immunity, impacting immune cell function.

More Related Videos

Isolation, Transfection, and Culture of Primary Human Monocytes
09:13

Isolation, Transfection, and Culture of Primary Human Monocytes

Published on: December 16, 2019

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
08:31

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Published on: October 18, 2016

Related Experiment Videos

Last Updated: Jun 17, 2026

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
07:35

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Published on: December 24, 2016

Isolation, Transfection, and Culture of Primary Human Monocytes
09:13

Isolation, Transfection, and Culture of Primary Human Monocytes

Published on: December 16, 2019

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
08:31

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Published on: October 18, 2016

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Dendritic cells (DCs) are crucial for initiating adaptive immune responses.
  • Hepatitis C virus (HCV) is known to evade host immune responses.
  • The interaction between HCV and DCs requires further elucidation.

Purpose of the Study:

  • To investigate the impact of Hepatitis C virus (HCV) on monocyte-derived dendritic cell (DC) function.
  • To assess the effect of HCV on DC maturation and type 1 interferon (IFN) production.
  • To explore potential mechanisms of viral immune evasion.

Main Methods:

  • Monocyte-derived DCs were cultured and exposed to JFH-1 (HCV genotype 2a) or Newcastle disease virus (NDV).
  • Real-time RT-PCR was used to assess viral replication and IFN expression.
  • Morphological changes were observed using light microscopy.
  • Immunophenotyping was performed by flow cytometry.

Main Results:

  • HCV did not replicate in or release particles from DCs.
  • HCV exposure induced a shift towards immature DCs, inversely correlated with viral titres.
  • HCV suppressed type 1 IFN (IFNβ) expression in DCs, diminishing NDV-induced IFNβ.
  • IFNα production was not affected by direct HCV contact.

Conclusions:

  • HCV negatively impacts DC maturation and suppresses their type 1 IFN response.
  • HCV appears to employ mechanisms to evade host immunity by targeting DCs.
  • These findings provide insights into viral immune evasion strategies.