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Updated: Jun 17, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
A L-cysteine sensor based on Pt nanoparticles/poly(o-aminophenol) film on glassy carbon electrode
Li-Ping Liu1, Zhao-Jing Yin, Zhou-Sheng Yang
1College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, Wuhu, PR China.
Abstract:
Platinum nanoparticles (nano-Pt) and poly(o-aminophenol) (POAP) were fabricated onto the glassy carbon electrode(GCE) to form the nano-Pt/POAP/GCE for the electrochemical determination of L-cysteine. The POAP film was obtained through electrochemical polymerization of o-aminophenol on GCE. The nano-Pt was electrochemically deposited onto the surface of the activated POAP/GCE The resultant nano-Pt/POAP/GCE was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and showed excellent electrochemical response to L-cysteine at low oxidative potential in Britton-Robinson (BR) buffer solution (pH=3.0), with good stability and sensitivity, and featured with a low detection limit (0.08 microM, signal/noise=3) and wide linear range (0.4 microM-6.3mM).The mechanism for the electrochemical oxidation of L-cysteine on the nano-Pt/POAP/GCE was also investigated.

