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Updated: Jun 17, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Phosphatidylinositol 3-kinase isoforms as targets in respiratory disease

John A Marwick1, Kian Fan Chung, Ian M Adcock

  • 1Section of Airways Disease, National Heart and Lung Institute, Imperial College London, London, UK. j.marwick@imperial.ac.uk

Therapeutic Advances in Respiratory Disease
|January 7, 2010
PubMed

Insights

Phosphoinositide 3-kinase (PI3K) signaling is crucial for immune responses in respiratory diseases like COPD and asthma. Targeting PI3Kgamma and PI3Kdelta may offer new therapeutic strategies for these conditions.

Area of Science:

  • Immunology
  • Pharmacology
  • Respiratory Medicine

Background:

  • Current therapies inadequately control chronic obstructive pulmonary disease (COPD), severe asthma, cystic fibrosis (CF), and idiopathic pulmonary fibrosis (IPF).
  • The molecular mechanisms and pathogenesis of these respiratory diseases are not fully understood, hindering the identification of new therapeutic targets.

Purpose of the Study:

  • To investigate the role of phosphoinositide 3-kinase (PI3K) signaling in the immune system and its potential as a therapeutic target for respiratory diseases.
  • To explore the specific involvement of leukocyte-specific PI3Kgamma and PI3Kdelta isoforms in inflammatory responses.

Main Methods:

  • Review of recent studies identifying PI3K as a central signaling mediator in immune responses.
  • Focus on pharmacologically targeting leukocyte-specific PI3Kgamma and PI3Kdelta isoforms.

Main Results:

  • PI3K plays an integral role in the initiation and maintenance of inflammatory responses.
  • PI3Kgamma and PI3Kdelta are central to leukocyte function and can be targeted pharmacologically.

Conclusions:

  • Early clinical studies using selective PI3K isoform inhibitors are necessary to evaluate their therapeutic potential in respiratory diseases.
  • Targeting PI3K may help control innate and adaptive inflammatory responses, restore glucocorticoid function, and reduce tumorigenesis in respiratory conditions.

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