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Cryomold: a device for tissue embedding in Mohs micrographic surgery

B Leshin1, S R Cook, D W Frye

  • 1Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina.

The Journal of Dermatologic Surgery and Oncology
|March 1, 1991
PubMed

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...